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Equal amounts of protein (30 μg) were loaded onto 10% SDS-PAGE gel, then electrophoresed and transferred to 0.2 μm PVDF membrane (Millipore, Darmstadt, Germany), which were blocked with 5% BSA for 2 h and incubated with the following primary antibodies overnight at 4°C: against COX-2 (1:1000), iNOS (1:2000), p-NF-κB p65 (1:1000), NF-κB P65 (1:1000), p-AKT (1:2000), AKT (1:2000), p-P38 (1:2000), P38 (1:1000), p-ERK1/2 (1:2000), ERK1/2 (1:2000), p-JNK (1:2000), JNK (1:2000), and β-actin (1:2000)
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As these technologies evolve, they offer the potential to redefine disease management across a broad spectrum of indications, setting the stage for the next generation of nanotechnology-enabled precision medicine
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