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Homogenates were centrifuged at 10,000 × g for 20 min at 4 °C, and supernatants were collected and subjected to hydrophobic chromatography (C18 SepPak cartridges, Waters Corporation) according to the manufacturer’s instructions

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Barbour: Time in range in pregnancy may need to be a lot higher, or tighter ranges may be required, to make a significant difference in pregnancy outcomes in type 2 diabetes or in gestational diabetes, perhaps because there are so many other contributors to fetal overgrowth