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For determination of plasma concentrations of semaglutide, 22 serial blood samples were drawn predose (–15 minutes) and 4, 8, 12, 16, 24, 28, 32, 36, 40, 48, 54, 60, 72, 96, 120, 168, 240, 336, 504, 672, and 840 hours after the last dose of semaglutide 1.0 mg (Figure 1)