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Materials and methods Tissues A total of 221 formalin-fixed human thyroid and pancreatic tissues (see Tables 1 and 2) were used in this study: normal thyroids ( n =8), thyroids with C-cell hyperplasia ( n =8), papillary thyroid carcinomas ( n =8), sporadic medullary thyroid carcinomas ( n =10), familial (MEN 2) medullary thyroid carcinomas ( n =10), chronic pancreatitis tissues ( n =9), benign insulinomas ( n =31), malignant insulinomas ( n =7), pancreatic nonfunctioning NET ( n =10), ductal pancreatic carcinomas ( n =88), PanIN 1/2 ( n =20), and PanIN 3 ( n =12)